“Methods for assessing the biological activity of soils and taxonomic diversity in environmental objects” - course 50,000 rubles. from MSU, training 4 weeks. (1 month), Date: November 29, 2023.
Miscellaneous / / December 02, 2023
Professor, Doctor of Biological Sciences, Honored Lecturer at Moscow University.
Biology and Soil Building of Moscow State University, room. 298 B
Graduated from the Faculty of Biology and Soil Sciences of Moscow State University in 1971. She defended her PhD thesis on the topic “Biology of psychrophilic coryne-like bacteria” (1978), her doctoral thesis “Bacterial communities of urban soils” (2010).
Area of scientific interests: soil microbiology, bacteria in extreme and anthropogenic (urban) habitats.
Main areas of research:
1. Studying the specifics of the formation and functioning of bacterial communities in extreme cold (Arctic, Antarctica) and desert (Karakum, Kyzylkum, Negev) habitats, urban soils (Moscow region).
2. Ecology of poorly studied genera of non-mycelial actinobacteria (Agromyces, Geodermatophilus).
3. Bacteria in poorly studied soil loci (hyphosphere, mycorrhizosphere, soil neoplasms).
4. Study of soil nanoforms of bacteria (ultramicrobacteria) - the smallest prokaryotic inhabitants of the soil.
The work is carried out using original methods developed in the laboratory (filtration through nuclear membrane filters, coloring dye L7012, which allows to characterize cell viability), the molecular biological method FISH and traditional soil methods bacteriology.
Reads special courses for soil scientists (“Soil Microbiology”) “Biochemistry of soil microorganisms”, “Structure, development and ecology of bacteria”, conducts seminars on the course “General Ecology” and laboratory classes on soil bacteriology and biochemistry. For ecologists (specialization “Biological control”) he teaches new original courses “Ecology of Prokaryotes” and “Ecological Biochemistry”.
She was awarded the “Veteran of Labor” and “In Memory of the 850th Anniversary of Moscow” medals.
Author of about 200 publications. Author and co-author of the textbooks “Methods for the isolation and identification of soil bacteria” (1989), “Gas chromatography methods in soil microbiology” (2002), “Bacterial diversity of soils and methods for its study” (2004), “Methodological materials for seminars on the course “General Ecology” (2003), “Assessment methods bacterial diversity of soils and identification of soil bacteria" (2003), "Methods for isolating and studying soil actinomycetes, producers of antibiotics" (2005), “General ecology. Methodological materials for seminar classes" (2008), "Methods for assessing the abundance and diversity of bacterial and actinomycete complexes of peat soils" (2010). Together with other employees of the department, chapters were written in collective monographs: “Structural and functional role of soil in the biosphere” (1999), “Ecology at the turn of the 21st century” century" (1999), "Soil degradation and protection" (2002), "Structural and functional role of soil and soil biota in the biosphere" (2003), "The role of soil in the formation and preservation of biological diversity" (2011), "Soils in the biosphere and human life: monograph" (2012), "Functioning of microbial complexes in high peat bogs: analysis of the causes of slow destruction peat" (2013).
1 Selection and preparation of soil samples and other types of substrates for microbiological studies
3 Gas chromatographic methods for determining the biological activity of soils and soils
3.1 Methods for determining the activity of microbiological processes of the nitrogen cycle: nitrogen fixation, denitrification
3.2 Methods for determining the activity of microbiological processes of the carbon cycle: methanogenesis and carbon dioxide emission.
3.3 Kinetic method for determining the biomass of microorganisms
4 Molecular biological methods for determining the metabolically active biomass of microorganisms, its functional orientation and taxonomic affiliation
5.1 Use of fluorescence microscopy to quantify microbial biomass and characterize its structure
5.2 Use of light optical microscopy to quantify microbial biomass and characterize its structure
5.3 Molecular biological methods for maintaining gene copies of different groups of microorganisms
5.4 Method for determining the functional metabolic diversity of microbial communities of soil, grounds and water (MST method)
6 Classical and modern molecular biological methods for taking into account the taxonomic and functional diversity of cultivated groups of the microbial population of soils, soils and related substrates
6.1 Methodological approaches to the identification of different trophic groups of microorganisms into selective ones nutrient media (seeding suspensions, lumps/fragments of substrates, glass fouling, isolation on bait)
6.2 Molecular biological methods for identifying microorganisms