“Biotesting in an accredited laboratory: standardization of test cultures” - course 60,000 rubles. from MSU, training 6 weeks. (1.5 months), Date: November 28, 2023.
Miscellaneous / / November 28, 2023
Doctor of Biological Sciences, Professor of the Department of Land Resources and Soil Assessment, Faculty of Soil Science, Moscow State University. Lomonosov.
She graduated with honors from the Faculty of Biology and graduate school at Moscow State University named after M. IN. Lomonosov.
She defended her doctoral dissertation “Bioindicative significance of micromycetes in the environmental assessment of aquatic and terrestrial ecosystems" (2004) and candidate's thesis "Study of specificity in the relationship between potato and its parasite Phytophthora infestans" (1980).
Area of scientific interests: biodiagnostics methodology, biodiversity, biomonitoring, microorganisms, micromycetes, ecotoxicity, remediation, mechanisms of resistance to pollutants, humates, bio- and nanotechnology
Organizer and head of the accredited laboratory for ecotoxicological analysis of soils (LETAP, https://www.letap-msu.ru/), on the basis of which applied and scientific projects are being implemented to determine the hazard class of waste from traditional and new technologies (nanomaterials, microplastics, pharmaceuticals), for assessing soil remediation, etc., including the author’s methods for measuring toxicity, recommended for practical use in environmental control.
Author and director of advanced training programs for ecologists in biotesting (Biotesting technologies...etc.).
Member of the editorial boards of scientific journals. Expert of the Russian Academy of Sciences, RINCCE and other foundations.
Delivers training courses related to biotic and interdisciplinary experimental assessment of environmental objects (Technologies for biotesting of soils and adjacent environments, Environmental impact assessment, Bioindication of pollution, Environmental regulation of the biological state of the environment and etc.)
Supervisor of qualifying works of undergraduate and graduate students, defended candidate dissertations (8). Author of more than 300 publications, monographs, and textbooks.
1 Basics of biotesting. The importance of the quality of test cultures for biotesting using standard methods.
1.1 Theoretical and practical aspects of laboratory biotesting. Modern biotest systems based on organisms of different taxonomic affiliations. Introduction to the main test functions and toxicity criteria.
1.2 Variety of standard biotests and principles for selecting test organisms. Sources of receipt. The influence of the quality of test cultures and experimental conditions on the result.
2.1 Biological features of microalgae as test organisms. Coenobial green algae Scenedesmus quadricauda. Polymorphic diatoms Phaeodactylum tricornutum. Development life cycle. Habitat and breeding conditions.
2.2 Biological features of crustaceans as test organisms. Characteristics of Daphnia magna, Ceriodaphnia affinis, Artemia salina. Development life cycle. Habitat and breeding conditions.
2.3 Biological and ecological characteristics of protozoa as test organisms. The structure of the ciliates Tetrahymena pyriformis and Paramecium caudatum. Development life cycle. Habitat and breeding conditions.
2.4 Genetically modified luminous bacteria: sources of production, storage conditions of the bacterial preparation "Ecolum", obtaining and testing the viability of a working suspension of E cells. coli
2.5 Higher plants. Quality of seeds of dicotyledonous and monocotyledonous plants (Sinapis alba, Avena sativa, etc.). Differences in seed weight. Assessment of phytoeffect in the Fitoscan system based on germination energy, length of roots and sprouts of seed sprouts.
2.6 Soil animals. Oligochaeta enchytraeidae (Oligochaeta, Enchytraeidae). Development life cycle. Habitat and breeding conditions.
3.1 Media for cultivation of crustaceans. Types of cultivation water. Methods for obtaining biologized fresh cultivation water for crustaceans. The composition of sea water is optimal for crustaceans. Cultivation environment update mode. Optimal parameters of temperature and illumination. Feeding regimen.
3.2 Media for the cultivation of microalgae. Preparation of nutrient media: Uspensky-1, Tamiya, Golberg media. Stock cultures of microalgae. Cultivation environment update mode. Optimal parameters of temperature and illumination.
3.3 Media for the cultivation of protozoa. Methods for obtaining nutrient solutions. Composition of the Lozin-Lozinsky medium. Cultivation environment update mode. Optimal parameters of temperature and illumination.
3.4 Solid substrates for plants and animals. Artificially prepared, reference and standard soils. ISO11268-2 standard. Optimal parameters of temperature, light, humidity.
4.1 Experimental testing of the sensitivity of freshwater microalgae Scenedesmus quadricauda to comparison toxicant based on changes in the number of algae cells and fluorescence intensity chlorophyll
4.2 Experimental testing of the sensitivity of marine microalgae Phaeodactylum tricornutum to a toxicant of comparison based on the intensity of chlorophyll fluorescence
4.3 Experimental testing of the sensitivity of freshwater crustaceans Daphnia magna and Ceriodaphnia affinis to a comparison toxicant based on survival
4.4 Experimental testing of the sensitivity of the brackish-water crustaceans Artemia salina to a comparison toxicant based on survival
4.5 Experimental testing of the sensitivity of protozoa - ciliates Paramecium caudatum to a toxicant of comparison in terms of survival
4.6 Assessment of phytoeffect in the Fitoscan system based on germination energy, length of roots and sprouts of seed sprouts.
Advanced training (16 hours). Economics and organization of construction. Program of additional professional education in tariff calculation and tariff regulation for current specialists in the field of electricity, heat supply, gas supply, water supply and drainage.
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